Effect of exogenous DNA microinjection on early development response of the seabream (Sparus aurata).
نویسندگان
چکیده
DNA transfer techniques allow genetic manipulation of commercial fish. However, marine species have received little attention because of their difficult zootechnical requirements. The seabream (Sparus aurata) has become one of the most important species in the aquaculture of Mediterranean countries, and the development of suitable DNA transfer procedures represents a main step in its genetic improvement. To assess the response of the seabream to exogenous DNA, naturally fertilized eggs were injected with the plasmids pCMV-CAT, pCMVTklacZ, and pEGFP-N1, in supercoiled and linearized forms. Embryo and larval survival, DNA fate, and reporter gene expression were analyzed during early development. The survival results indicate that microinjection is an effective transfer method in spite of the unfavorable conditions. Linearized plasmids were more efficiently polymerized than supercoiled ones; however, no significant differences were detected either in their persistence or in their expression levels. Reporter gene expression was initiated after mid-blastula transition. The duration of transient expression varied between the promoter-gene combinations, and no integration of transgenes into fish chromosomes was detected. Results suggest that the main factor affecting the persistence and expression of DNA seems to be related to developmental processes. Among the markers used, CAT proved to be the most sensitive, but GFP had obvious methodologic advantages over the spatial marker lacZ. The usefulness of GFP for diagnosis of transgenesis is enhanced by the transparency of embryos and larvae in S. aurata.
منابع مشابه
Growth and reproduction studies on gilhead seabream (Sparus aurata) in Beymelek Lagoon, Turkey
In the present study, age, growth, length-weight relationship and reproduction were investigated in gilthead seabream (Sparus aurata L., 1758) collected from Beymelek Lagoon (Antalya, Turkey) between February 2006 and July 2007. The age, total length and weight of samples ranged from 0+ to 4 years, 10.6 to 35.5cm, and 18 to 928g, respectively. Growth was described by the standard form of the vo...
متن کاملResponse of Gilthead Seabream (Sparus aurata L., 1758) Larvae to Nursery Odor Cues as Described by a New Set of Behavioral Indexes
Citation: Morais P, Parra MP, Baptista V, Ribeiro L, Pousão-Ferreira P and Teodósio MA (2017) Response of Gilthead Seabream (Sparus aurata L., 1758) Larvae to Nursery Odor Cues as Described by a New Set of Behavioral Indexes. Front. Mar. Sci. 4:318. doi: 10.3389/fmars.2017.00318 Response of Gilthead Seabream (Sparus aurata L., 1758) Larvae to Nursery Odor Cues as Described by a New Set of Behav...
متن کاملGrowth and reproduction studies on gilhead seabream (Sparus aurata) in Beymelek Lagoon, Turkey
In the present study, age, growth, length-weight relationship and reproduction were investigated in gilthead seabream (Sparus aurata L., 1758) collected from Beymelek Lagoon (Antalya, Turkey) between February 2006 and July 2007. The age, total length and weight of samples ranged from 0+ to 4 years, 10.6 to 35.5cm, and 18 to 928g, respectively. Growth was described by the standard form of the vo...
متن کاملThe complete mitochondrial genome of the gilthead seabream Sparus aurata L. (Sparidae).
The complete mitochondrial genome of the gilthead seabream Sparus aurata Linnaeus 1758, one of the world's most important mariculture species, was sequenced using next generation sequencing technology. The genome sequence is comprised of 16,652 bp exhibiting the canonical vertebrate mitochondria gene order. Regions of gene overlap, tRNA length, as well as start and stop codon were similar to th...
متن کاملThe first comparative study on the growth performance of European seabass (Dicentrarchus labrax, L. 1758) and gilthead seabream (Sparus aurata, L. 1758) commercially farmed in low salinity brackish water and earthen ponds
In this study, 50 000 European seabass and 50 000 gilthead seabream with an average wet weight of 1.6 g were cultivated during 600 days under low water salinity conditions (7‰) of which getting out from underground at fixed 19°C, being produced in earthen ponds under commercial production conditions. From the beginning to the end of the experiment, the same fish feeds were used for both species...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Molecular marine biology and biotechnology
دوره 7 4 شماره
صفحات -
تاریخ انتشار 1998